Coding

Part:BBa_K4453001:Design

Designed by: Hugo Salas Pedroarena   Group: iGEM22_UPNAvarra_Spain   (2022-10-02)


Human TDC optimized for E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 339
    Illegal SpeI site found at 1507
    Illegal PstI site found at 1521
    Illegal NotI site found at 7
    Illegal NotI site found at 1514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 360
    Illegal XhoI site found at 870
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1507
    Illegal PstI site found at 1521
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K4453001 has been designed so that there is no restriction site inside it for the restrictions enzymes used in the prefixes nor suffixes for the Biobrick Assembly Method


Source

Retrieved form Part BBa_K399901. Optimized for its synthesis in E. coli via codon optimization software by IDT Technologies.

References

Sumi-Ichinose, C., Ichinose, H., Takahashi, E., Hori, T., & Nagatsu, T. (1992). Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and serotonin biosynthesis. Biochemistry, 31(8), 2229–2238. https://doi.org/10.1021/bi00123a004