Coding
Part:BBa_K4453001:Design
Designed by: Hugo Salas Pedroarena Group: iGEM22_UPNAvarra_Spain (2022-10-02)
Human TDC optimized for E. coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1506 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 339
Illegal SpeI site found at 1507
Illegal PstI site found at 1521
Illegal NotI site found at 7
Illegal NotI site found at 1514 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal XhoI site found at 360
Illegal XhoI site found at 870 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 1507
Illegal PstI site found at 1521 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Part BBa_K4453001 has been designed so that there is no restriction site inside it for the restrictions enzymes used in the prefixes nor suffixes for the Biobrick Assembly Method
Source
Retrieved form Part BBa_K399901. Optimized for its synthesis in E. coli via codon optimization software by IDT Technologies.
References
Sumi-Ichinose, C., Ichinose, H., Takahashi, E., Hori, T., & Nagatsu, T. (1992). Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and serotonin biosynthesis. Biochemistry, 31(8), 2229–2238. https://doi.org/10.1021/bi00123a004